RNA

Part:BBa_K864440:Design

Designed by: Hampus Elofsson, Sabri Jamal, Lisa Branzell   Group: iGEM12_Uppsala   (2012-09-25)


Small RNA spot42


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It can be a bit tricky to do mutagenesis on it, like we did. We wanted to add a a random antisense area with primers. You cannot have any scars between the promotor and sRNA so we added our promotor with mutagenesis.



Source

It was biobricked from E-coli MG1655

References