RNA
Part:BBa_K864440:Design
Designed by: Hampus Elofsson, Sabri Jamal, Lisa Branzell Group: iGEM12_Uppsala (2012-09-25)
Small RNA spot42
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It can be a bit tricky to do mutagenesis on it, like we did. We wanted to add a a random antisense area with primers. You cannot have any scars between the promotor and sRNA so we added our promotor with mutagenesis.
Source
It was biobricked from E-coli MG1655